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Calculator
Tris-Glycine Transfer Buffer is used as a transfer buffer in Western blotting.
To prepare L ofTris-Glycine Transfer Buffer (20x):
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
| Component | Amount | Concentration |
| Tris base (mw: 121.14 g/mol) | 24.2 g | 0.2 M |
| Glycine (mw: 75.07 g/mol) | 150.1 g | 2 M |
- Prepare 800 mL of distilled water in a suitable container.
- Add 24.2 g of Tris base to the solution.
- Add 150.1 g of Glycine to the solution.
- Add distilled water until the volume is 1 L.
- pH adjustment is not necessary (it will be ~8.8). Store at room temperature.
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Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis
References
This online tool may be cited as follows
MLA | "QuestCalculate™Tris-Glycine Transfer Buffer (20x) Preparation and Recipe."AAT Bioquest, Inc.,7Apr.2024,https://www.aatbio.com/resources/buffer-preparations-and-recipes/tris-glycine-transfer-buffer-20x. | |
APA | AAT Bioquest, Inc. (2024,April7).QuestCalculate™Tris-Glycine Transfer Buffer (20x) Preparation and Recipe. AAT Bioquest.https://www.aatbio.com/resources/buffer-preparations-and-recipes/tris-glycine-transfer-buffer-20x. | |
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